Constitutive synthesis of activator protein 1 transcription factor after viral transformation of mouse fibroblasts

Journal Article · Sun May 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.10.3401· OSTI ID:5599233

Piette, J; Hirai, S I; Yaniv, M ^[1]

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1. Centre National de la Recherche Scientifique, Paris (France)

Transcription factor activator protein 1 (AP1) interacts with the promoter region of a number of genes that are stimulated by growth factors present in serum or by agents such as phorbol 12-myristate 13-acetate (PMA) that partially mimic their action. To investigate the possible role of AP1 in the control of cellular growth and in transformation of mammalian cells, the authors monitored its activity by binding to a specific DNA probe in normal or transformed mouse NIH 3T3 cells. A 3- to 4-fold increase in the DNA-binding activity was found after serum stimulation of quiescent NIH 3T3 cells. A 2- to 3-fold activation was found after treatment with PMA or dibutyryl-cAMP, suggesting that different signal-transducing pathways could activate AP1 factor in these cells. PMA stimulation was dependent on new protein synthesis. In contrast to normal cells, a high serum-independent AP1 DNA-binding activity was found in NIH 3T3 cells transformed by simian virus 40. These results suggest that constitutive AP1 synthesis may be a crucial step in cellular transformation.

• OSTI ID: 5599233
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:10; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARCINOGENS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
ESTERS
FIBROBLASTS
GENE REPRESSORS
GENES
GROWTH FACTORS
MICROORGANISMS
MITOGENS
NUCLEOPROTEINS
ONCOGENES
ORGANIC COMPOUNDS
PARASITES
PHORBOL ESTERS
PROTEINS
SIMIAN VIRUS
SOMATIC CELLS
SYNTHESIS
TRANSCRIPTION
VIRUSES