Stability of DNA in mammalian cells irradiated with near-uv light (uv-a)
Journal Article
·
· Radiat. Res.; (United States)
The induction of lesions in human melanoma DNA by near-uv light (uv-a) has been investigated using a procedure to lyse the cells in dilute alkali. The alkaline treatment denatures the DNA, which results in fragmentation of the DNA in irradiated cells but not in control cells. The solution is neutralized to allow the larger fragments to renature. The smaller, single-stranded DNA fragments (2-10 kb) are then detected using agarose gel electrophoresis. With increasing dose of irradiation there are increasing amounts of the smaller fragments detected. The DNA fragmentation, which can be prevented by treating the cells with the radical scavenger DMSO, is not induced via the incubation medium. Hence uv-a has distinct effects on chromosomal DNA.
- Research Organization:
- Karolinska Hospital, Stockholm, Sweden
- OSTI ID:
- 6470966
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 99:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Formation of a new population of DNA replication intermediates in X-irradiated human melanoma cells
Formation of a new population of DNA replication intermediates in X-irradiated human melanoma cells
Radioimmune assay of induction and removal of uv lesions in total and staphylococcal nuclease-resistant DNA of mammalian chromatin
Journal Article
·
Thu Dec 31 23:00:00 EST 1981
· Carcinogenesis (N.Y.); (United States)
·
OSTI ID:6578345
Formation of a new population of DNA replication intermediates in X-irradiated human melanoma cells
Journal Article
·
Thu Dec 31 23:00:00 EST 1981
· Carcinogenesis (N.Y.); (United States)
·
OSTI ID:6490917
Radioimmune assay of induction and removal of uv lesions in total and staphylococcal nuclease-resistant DNA of mammalian chromatin
Journal Article
·
Sun Jan 31 23:00:00 EST 1982
· Radiat. Res.; (United States)
·
OSTI ID:5579586
Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DATA
DISEASES
DMSO
DNA
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EXPERIMENTAL DATA
INFORMATION
MELANOMAS
NEAR ULTRAVIOLET RADIATION
NEOPLASMS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SCAVENGING
STRAND BREAKS
SULFOXIDES
TUMOR CELLS
ULTRAVIOLET RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DATA
DISEASES
DMSO
DNA
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EXPERIMENTAL DATA
INFORMATION
MELANOMAS
NEAR ULTRAVIOLET RADIATION
NEOPLASMS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SCAVENGING
STRAND BREAKS
SULFOXIDES
TUMOR CELLS
ULTRAVIOLET RADIATION