Formation of a new population of DNA replication intermediates in X-irradiated human melanoma cells

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.7.829· OSTI ID:6490917

Loenn, U ^[1]

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1. Kungliga Karolinska Mediko-Kirurgiska Inst., Stockholm (Sweden)

DNA synthesis was investigated in human melanoma cells irradiated with X-rays. The cells were lysed in dilute alkali which results in the release from the parental DNA of replication intermediates; these are then analyzed by agarose gel electrophoresis. In cells irradiated with 10Gy the formation of a new heterogeneous population of DNA replication intermediates with a size >10kb was detected. The presence of other DNA replication intermediates were also demonstrated in control cells. The results imply that in melanoma cells changes may occur in the replication in order to comply with alterations in the DNA induced by X-rays.

• OSTI ID: 6490917
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:7; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DISEASES
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MAN
MELANOMAS
NEOPLASMS
NUCLEIC ACID REPLICATION
PRIMATES
RADIATION EFFECTS
RADIATIONS
TUMOR CELLS
VERTEBRATES
X RADIATION