Formation of a new population of DNA replication intermediates in X-irradiated human melanoma cells

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.7.829· OSTI ID:6578345

Loenn, U

DNA synthesis was investigated in human melanoma cells irradiated with X-rays. The cells were lysed in dilute alkali which results in the release from the parental DNA of replication intermediates; these are then analyzed by agarose gel electrophoresis. In cells irradiated with 10 Gy we can detect the formation of a new heterogeneous population of DNA replication intermediates with a size greater than 10 kb. We can also demonstrate the presence of other DNA replication intermediates which were earlier described in control cells. The results imply that in melanoma cells changes may occur in the replication in order to comply with alterations in the DNA induced by X-rays.

• Research Organization: Department of Histology Karolinska Instituet, Stockholm, Sweden
• OSTI ID: 6578345
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:7; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
DISEASES
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
IONIZING RADIATIONS
MELANOMAS
NEOPLASMS
NUCLEIC ACID REPLICATION
RADIATIONS
REACTION INTERMEDIATES
TUMOR CELLS
X RADIATION