Radioimmune assay of induction and removal of uv lesions in total and staphylococcal nuclease-resistant DNA of mammalian chromatin
We have used a radioimmune assay (RIA) to measure the induction and removal of uv lesions in total (DNA) and staphylococcal nuclease-resistant DNA (DNA) of nuclei from uv-irradiated mammalian cells. Antibodies prepared against uv-irradiated single-stranded DNA were specific for uv-irradiated DNA. In Chinese hamster ovary (CHO) cells uv lesion induction was shown by RIA to be linear with dose in the range of 20 to 80 J/m/sup 2/. Lesion induction per unit DNA in the DNA/sub r/ of nuclei extracted from CHO and HeLa cells irradiated with 7 and 15 J/m/sup 2/ was 80% of the induction in the DNA/sub t/. This difference is insufficient to account for the two- to fourfold difference of repair synthesis reported for these fractions. There was no detectable removal of the lesions induced by 15 J/m/sub 2/ in CHO cells in 24 hr of repair incubation. In HeLa cells, however, 40 and 50% of the lesions induced by 15 J/m/sup 2/ of uv light were removed in 6 and 24 hr of repair incubation, respectively. Although the production of lesions is lower in DNA, than in DNA/sub t/, at 4 and 6 hr of repair incubation there are approximately equal numbers of lesions in these DNAs of HeLa cells. This can be explained by (1) higher rate of removal of lesions in staphylococcal nuclease-sensitive DNA (DNAs) or (2) nucleosomal rearrangement during repair.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 5579586
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 89:2; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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560152 -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CHROMATIN
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
FEMALE GENITALS
GONADS
HAMSTERS
HELA CELLS
HYDROLASES
INDUCTION
ISOTOPE APPLICATIONS
MAMMALS
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OVARIES
PHOSPHODIESTERASES
RADIATION EFFECTS
RADIATIONS
RADIOASSAY
RADIOIMMUNOASSAY
REMOVAL
RODENTS
STAPHYLOCOCCUS
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
VERTEBRATES