Inhibition by hyperthermia of repair synthesis and chromatin reassembly of ultraviolet-induced damage to DNA

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576524· OSTI ID:6024294

Bodell, W J; Cleaver, J E; Roti Roti, J L

The authors have investigated the effects of hyperthermia treatment on sequential steps of the repair of UV-induced DNA damage in HeLa cells. DNA repair synthesis was inhibited by 40% after 15 min of hyperthermia treatment at 45/sup 0/C; greater inhibition of repair synthesis occurred with prolonged incubation at 45/sup 0/C. Enzymatic digestion of repair-labeled DNA with Exonuclease III indicated that once DNA repair was initiated, the DNA repair patch was synthesized to completion and that ligation of the DNA repair patch occurred. Thus, the observed inhibition of UV-induced DNA repair synthesis by hyperthermia treatment may be the result of inhibition of enzymes involved in the initiating steps(s) of DNA repair. DNA repair patches synthesized in UV-irradiated cells labeled at 37/sup 0/C with(/sup 3/H)Thd were 2.2-fold more sensitive to micrococcal nuclease digestion than was parental DNA; if the length of the labeling period was prolonged, the nuclease sensitivity of the repair patch synthesized approached that of the parental DNA. DNA repair patches synthesized at 45/sup 0/C, however, remained sensitive to micrococcal nuclease digestion even after long labeling periods, indicating that heat treatment inhibits the reassembly of the DNA repair patch into nucleosomal structures. 23 references, 3 figures, 2 tables.

• Research Organization: Univ. of California, San Francisco
• DOE Contract Number: AC03-76SF01012
• OSTI ID: 6024294
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 100; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY TEMPERATURE
CHROMATIN
DATA
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
EXPERIMENTAL DATA
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HELA CELLS
HYDROLASES
HYPERTHERMIA
INFORMATION
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEASES
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION