Bent DNA functions as a replication enhancer in Saccharomyces cerevisiae

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.7.2763· OSTI ID:6470827

Williams, J S; Eckdahl, T T; Anderson, J N

Previous studies have demonstrated that bent DNA is a conserved property of Saccharomyces cerevisiae autonomously replicating sequences (ARSs). Hence they showed that bending elements are contained within ARS subdomains identified by others as replication enhancers. To provide a direct test for the function of this unusual structure, we analyzed the ARS activity of plasmids that contained synthetic bent DNA substituted for the natural bending element in yeast ARS1. The results demonstrated that deletion of the natural bending locus impaired ARS activity which was restored to a near wild-type level with synthetic bent DNA. Since the only obvious common features of the natural and synthetic bending elements are the sequence patterns that give rise to DNA bending, the results suggest that the bent structure per se is crucial for ARS function.

• Research Organization: Dept. of Biological Sciences, Purdue Univ., West Lafayette, IN (US)
• OSTI ID: 6470827
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:7; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
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550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
CHROMOSOME LOSSES
DNA REPLICATION
DNA SEQUENCING
EUMYCOTA
FUNGI
GENE OPERONS
GENETIC MAPPING
LOSSES
MAPPING
MICROORGANISMS
NUCLEIC ACID REPLICATION
PATTERN RECOGNITION
PLANTS
PLASMIDS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRUCTURAL CHEMICAL ANALYSIS
STRUCTURE-ACTIVITY RELATIONSHIPS
YEASTS