Identification of a 450-bp region of human papillomavirus type 1 that promotes episomal replication in Saccharomyces cerevisiae

Journal Article · Thu Sep 15 04:00:00 EDT 2005 · Virology

DOI:https://doi.org/10.1016/j.virol.2005.06.029· OSTI ID:20729131

Chattopadhyay, Anasuya ^[1]; Schmidt, Martin C ^[1]; Khan, Saleem A ^[1]

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1. Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Room East 1240 Biomedical Science Tower, Pittsburgh, PA 15261 (United States)

Human papillomaviruses (HPVs) replicate as nuclear plasmids in infected cells. Since the DNA replication machinery is generally conserved between humans and Saccharomyces cerevisiae, we studied whether HPV-1 DNA can replicate in yeast. Plasmids containing a selectable marker (with or without a yeast centromere) and either the full-length HPV-1 genome or various regions of the viral long control region (LCR) and the 3' end of the L1 gene were introduced into S. cerevisiae and their ability to replicate episomally was investigated. Our results show that HPV-1 sequences promote episomal replication of plasmids although the yeast centromere is required for plasmid retention. We have mapped the autonomously replicating sequence activity of HPV-1 DNA to a 450 base-pair sequence (HPV-1 nt 6783-7232) that includes 293 nucleotides from the 5' region of the viral LCR and 157 nucleotides from the 3' end of the L1 gene. The HPV-1 ARS does not include the binding sites for the viral E1 and E2 proteins, and these proteins are dispensable for replication in S. cerevisiae.

• OSTI ID: 20729131
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 340; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CENTROMERES
DNA
DNA REPLICATION
LOAD COLLECTOR RATIO
NUCLEOTIDES
PLASMIDS
PROTEINS
SACCHAROMYCES CEREVISIAE