Isolation and subcloning analysis of functional centromere DNA(CEN11) from Saccharomyces cerevisiae chromosome XI

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.1.82· OSTI ID:5652999

Fitzgerald-Hayes, M; Buhler, J M; Cooper, T G; Carbon, J

The authors have cloned segments of yeast DNA containing the centromere XI-linked MET14 gene. This was done by selecting directly in Saccharomyces cerevisiae for complementation of a met14 mutation after transformation with a hybrid plasmid DNA genomic library. Generic evidence indicates that functional centromere DNA (CEN11) from chromosome XI is also contained on the segment of S. cerevisiae DNA cloned in pYe(MET14)2. This plasmid is maintained stably in budding S. cerevisiae cultures and segregates predominantly 2 + :2- through meiosis. The CEN11 element has been subcloned in vector YRp7' on an S. cerevisiae DNA fragment 900 base pairs in length (pYe(CEN11)10). The mitotic and meiotic behavior of plasmids containing CEN11 plus a DNA replicator (ars) indicates that the centromere DNA sequences enable these plasmids to function as true minichromosomes in S. cerevisiae.

• Research Organization: Dept. of Biological Sciences, Univ. of California, Santa Barbara, CA 93106
• OSTI ID: 5652999
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:1; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CELL CONSTITUENTS
CELL CULTURES
CELL DIVISION
CELL TRANSFORMATIONS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DNA REPLICATION
DNA-CLONING
EXTRACTION
FUNGI
GENES
GENOME MUTATIONS
HYBRIDOMAS
MEIOSIS
MICROORGANISMS
MITOSIS
MUTATIONS
NUCLEIC ACID REPLICATION
ONCOGENIC TRANSFORMATIONS
PHYSIOLOGY
PLANTS
PLASMIDS
REDUCTIVE EXTRACTION
REPLICONS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SEPARATION PROCESSES
STRUCTURE-ACTIVITY RELATIONSHIPS
YEASTS