Isolation and subcloning analysis of functional centromere DNA(CEN11) from Saccharomyces cerevisiae chromosome XI
The authors have cloned segments of yeast DNA containing the centromere XI-linked MET14 gene. This was done by selecting directly in Saccharomyces cerevisiae for complementation of a met14 mutation after transformation with a hybrid plasmid DNA genomic library. Generic evidence indicates that functional centromere DNA (CEN11) from chromosome XI is also contained on the segment of S. cerevisiae DNA cloned in pYe(MET14)2. This plasmid is maintained stably in budding S. cerevisiae cultures and segregates predominantly 2 + :2- through meiosis. The CEN11 element has been subcloned in vector YRp7' on an S. cerevisiae DNA fragment 900 base pairs in length (pYe(CEN11)10). The mitotic and meiotic behavior of plasmids containing CEN11 plus a DNA replicator (ars) indicates that the centromere DNA sequences enable these plasmids to function as true minichromosomes in S. cerevisiae.
- Research Organization:
- Dept. of Biological Sciences, Univ. of California, Santa Barbara, CA 93106
- OSTI ID:
- 5652999
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 2:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PLASMIDS
PHYSIOLOGY
STRUCTURE-ACTIVITY RELATIONSHIPS
SACCHAROMYCES CEREVISIAE
CELL CULTURES
CHROMOSOMAL ABERRATIONS
GENOME MUTATIONS
CHROMOSOMES
DNA REPLICATION
DNA-CLONING
HYBRIDOMAS
MEIOSIS
MITOSIS
ONCOGENIC TRANSFORMATIONS
REDUCTIVE EXTRACTION
REPLICONS
ANIMAL CELLS
CELL CONSTITUENTS
CELL DIVISION
CELL TRANSFORMATIONS
CLONING
EXTRACTION
FUNGI
GENES
MICROORGANISMS
MUTATIONS
NUCLEIC ACID REPLICATION
PLANTS
SACCHAROMYCES
SEPARATION PROCESSES
YEASTS
550400* - Genetics