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Cloning of Saccharomyces cerevisiae gene conferring cadmium resistance

Journal Article · · Sov. Genet. (Engl. Transl.); (United States)
OSTI ID:5896495
; ;
A gene conferring resistance to the toxic effect of cadmium ions was cloned out of a Saccharomyces cerevisiae gene library based on vector pL3. Cadmium resistance was phenotypically expressed only if the CAD1 gene was present in a multicopy plasmid. Intrachromosomal integration of the plasmid or insertion of the centromeric region into the plasmid lowered the number of CAD1-gene copies and, correspondingly, the resistance to cadmium ions. The cloned, Sau3A-cleaved fragment of the yeast chromosome was 3.5 kg in size. Restriction analysis and subcloning of different portions of this DNA stretch determined the minimal fragment responsible for the Cad/sup r/ phenotype: the CAD1 gene was found to be located on an XhoI-Sau3A fragment 1.6 kb long. In Escherichia coli, instability of a yeast-DNA region adjacent to the XhoI-Sau3A fragment was observed.
Research Organization:
Scientific-Industrial Association ''Ferment'', Vilnyus (USSR)
OSTI ID:
5896495
Journal Information:
Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 24:5; ISSN SOGEB
Country of Publication:
United States
Language:
English

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Related Subjects

550300 -- Cytology
550400* -- Genetics
550700 -- Microbiology
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE PHOSPHATASE
BIOLOGICAL EFFECTS
CADMIUM COMPOUNDS
CADMIUM SULFATES
CELL CONSTITUENTS
CELL TRANSFORMATIONS
CLONING
CONTROL
DNA
DNA HYBRIDIZATION
DNA POLYMERASES
DNA-ASE
DNA-CLONING
ENDONUCLEASES
ENZYME ACTIVITY
ENZYMES
ESTERASES
EUMYCOTA
FUNGI
GENES
GENETIC CONTROL
GENETIC MAPPING
HYBRIDIZATION
HYDROLASES
LIGASES
MAPPING
METALLOPROTEINS
METALLOTHIONEIN
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEST CONTROL
PHENOTYPE
PHOSPHATASES
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
PLASMIDS
POLYMERASES
PROTEINS
RECOMBINANT DNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STABILITY
SULFATES
SULFUR COMPOUNDS
TRANSFERASES
YEASTS