Cloning and mapping of Saccharomyces cerevisiae photoreactivation gene PHR1

Journal Article · Sat Sep 01 04:00:00 EDT 1984 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.4.9.1864· OSTI ID:5267362

Schild, D; Johnston, J; Chang, C; Mortimer, R K

The yeast Saccharomyces cerevisiae, like most organisms, is able to directly repair pyrimidine dimers by using a photoreactivating enzyme and visible light. Cells carrying the phr1 mutation were shown previously to be unable to photoreactivate dimers, but neither the map position nor the primary gene product of the PHR1 gene has been determined. The authors have cloned this gene and determined its map position. A plasmid containing a 6.4 kilobase yeast DNA insert has been isolated and shown to restore photoreactivation in a phr1 strain. A 3.1-kilobase subclone has also been shown to complement phr1. The original plasmid was targeted to integrate into chromosomal DNA at a site homologous to the insert by cutting within the insert. Two of these integrants have been mapped on the right arm of chromosome XV; the integrants have been further mapped at ca. 13 centimorgans from prt1. It has also been independently determined that phr1 maps at this location. Thus, the map position of PHR1 has been determined and it has been shown that the plasmid contains PHR1 rather than a suppressor of the phr1 mutation. 34 references, 4 figures, 3 tables.

• Research Organization: Lawrence Berkeley Lab., CA
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5267362
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:9; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CHROMOSOMES
CLONING
DATA
DNA REPAIR
DNA-CLONING
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FUNGI
GENES
GENETIC MAPPING
INFORMATION
MAPPING
MICROORGANISMS
NUMERICAL DATA
PHOTOREACTIVATION
PLANTS
PLASMIDS
PYRIMIDINE DIMERS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SURVIVAL CURVES
VISIBLE RADIATION
YEASTS