Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.1.210· OSTI ID:7077494

Buchman, A R; Kimmerly, W J; Rine, J; Kornberg, R D

Two DNA-binding factors from Saccharomyces cerevisiae have been characterized, GRFI (general regulatory factor I) and ABFI (ARS-binding factor I), that recognize specific sequences within diverse genetic elements. GRFI bound to sequences at the negative regulatory elements (silencers) of the silent mating type loci HML E and HMR E and to the upstream activating sequence (UAS) required for transcription of the MAT ..cap alpha.. genes. A putative conserved UAS located at genes involved in translation (RPG box) was also recognized by GRFI. In addition, GRFI bound with high affinity to sequences within the (C/sub 1-3/A)-repeat region at yeast telomeres. Binding sites for GRFI with the highest affinity appeared to be of the form 5'-(A/G)(A/C)ACCCAN NCA(T/C)(T/C)-3', where N is any nucleotide. ABFI-binding sites were located next to autonomously replicating sequences (ARSs) at controlling elements of the silent mating type loci HMR E, HMR I, and HML I and were associated with ARS1, ARS2, and the 2..mu..m plasmid ARS. Two tandem ABFI binding sites were found between the HIS3 and DED1 genes, several kilobase pairs from any ARS, indicating that ABFI-binding sites are not restricted to ARSs. The sequences recognized by AFBI showed partial dyad-symmetry and appeared to be variations of the consensus 5'-TATCATTNNNNACGA-3'. GRFI and ABFI were both abundant DNA-binding factors and did not appear to be encoded by the SIR genes, whose product are required for repression of the silent mating type loci. Together, these results indicate that both GRFI and ABFI play multiple roles within the cell.

• Research Organization: Dept. of Cell Biology, Stanford Univ. School of Medicine, Stanford, CA (US)
• OSTI ID: 7077494
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:1; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
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59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ANALYSIS
BIOCHEMICAL REACTION KINETICS
CHEMICAL ANALYSIS
DNA REPLICATION
DNA SEQUENCING
FUNGI
GENE OPERONS
GENE REGULATION
GENE REPRESSORS
GENETIC MAPPING
KINETICS
MAPPING
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
REACTION KINETICS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS
YEASTS