Induction of sarcomas in nude mice by implantation of Syrian hamster fetal cells exposed in vitro to nickel subsulfide

Journal Article · Sat Sep 01 04:00:00 EDT 1979 · Cancer Res.; (United States)

OSTI ID:6459101

Costa, M ^[1]; Nye, J S; Sunderman, Jr, F W; Allpass, P R; Gondos, B

---

1. Univ. of Connecticut, Storrs

In vitro exposure of Syrian hamster fetal cells to nickel subsulfide (..cap alpha..Ni/sub 3/S/sub 2/) yielded positive colony assays for morphological transformation. A dose-response relationship was found between the concentration of ..cap alpha..Ni/sub 3/S/sub 2/ and the incidence of morphological transformation. Exposures of ..cap alpha..Ni/sub 3/S/sub 2/ induced morphological transformation at concentrations (0.1 or 1.0 ..mu..g/ml of culture medium) which did not impair cell plating efficiency. Nickel monosulfide (NiS) did not induce morphological transformation of Syrian hamster fetal cells under the same conditions. Clones of ..cap alpha..Ni/sub 3/S/sub 2/-transformed cells were able to grow in soft agar medium and demonstrated increased basal and induced activities of ornithine decarboxylase. Undifferentiated sarcomas developed in 26 of 27 nude mice at the site of s.c. injection of clones of ..cap alpha..Ni/sub 3/S/sub 2/-transformed cells. No tumors developed in 19 control nude mice which were given s.c. injections of nontransformed Syrian hamster fetal cells which had not been exposed to ..cap alpha..Ni/sub 3/S/sub 2/. This study demonstrates that fetal cells which undergo transformation following exposure to ..cap alpha..Ni/sub 3/S/sub 2/ are capable of producing malignant tumors in nude mice.

• OSTI ID: 6459101
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
CARCINOGENESIS
CHALCOGENIDES
CLONING
DISEASES
ENZYME ACTIVITY
FETUSES
HAMSTERS
IMPLANTS
IN VITRO
MAMMALS
MICE
MORPHOLOGY
NEOPLASMS
NICKEL COMPOUNDS
NICKEL SULFIDES
PATHOGENESIS
QUANTITY RATIO
RODENTS
SARCOMAS
SENSITIVITY
SULFIDES
SULFUR COMPOUNDS
TOXICITY
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES