Induction of ocular neoplasms in Fischer rats by intraocular injection of nickel subsulfide

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · Invest. Ophthalmol.; (United States)

OSTI ID:6578564

Albert, D M; Gonder, J R; Papale, J; Craft, J L; Dohlman, H G; Reid, M C; Sunderman, Jr, F W

Nickel subsulfide, ..cap alpha..Ni/sub 3/S/sub 2/, was administered to albino Fischer rats by a single injection into the vitreous body of the right eye (0.5 mg ..cap alpha..Ni/sub 3/S/sub 2//rat, suspended in 20 ..mu..l of NaCl vehicle). Control rats received a similar injection of the vehicle. Malignant tumors developed in the injected eyes of 14/15 ..cap alpha..Ni/sub 3/S/sub 2/-treated rats by 8 months (vs. 0/11 controls, p < 0.001). Five of the injected eyes of ..cap alpha..Ni/sub 3/S/sub 2/-treated rats contained multiple tumors. The 21 eye tumors that were induced by ..cap alpha..Ni/sub 3/S/sub 2/ included 11 nuclanomas, four retinoblastomas, three gliomas, and three unclassified malignant neoplasms. Three of the melanomas developed extraocular extensions; one of the melanomas metastasized to lungs and brain. Although the melanomas arose from amelanotic uveal melanocytes, melanosomes were observed in electron micrographs of the tumor cells. This study provides a new experimental model for chemical induction of ocular neoplasms. As a procedure to test the carcinogenicity of nickel compounds, intraocular injection has the advantages of short lateney period, high tumor incidence, and case of tumor detection.

• Research Organization: Massachusetts Eye and Ear Infirmary, Boston
• OSTI ID: 6578564
• Journal Information: Invest. Ophthalmol.; (United States), Journal Name: Invest. Ophthalmol.; (United States) Vol. 22:6; ISSN INOPA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism &amp; Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BODY
BODY AREAS
CARCINOGENESIS
CHALCOGENIDES
DISEASES
EYES
FACE
HEAD
INJECTION
INTAKE
LATENCY PERIOD
MAMMALS
METASTASES
NEOPLASMS
NICKEL COMPOUNDS
NICKEL SULFIDES
ORGANS
PATHOGENESIS
RATS
RODENTS
SENSE ORGANS
SENSITIVITY
SULFIDES
SULFUR COMPOUNDS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES