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Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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The repair of DNA double-strand breaks in Escherichia coli cells irradiated with ..gamma.. rays occurs only after new proteins are synthesized in response to damage introduced in the genome DNA. One protein whose synthesis is thus induced is the recA protein, and previous work has shown that recA/sup -/ cells do not repair double-strand breaks. However, inducing recA protein by treating cells with nalidixic acid does not induce repair of double-strand breaks, so this repair requires more than the presence of the recA protein. When repair of double-strand breaks is blocked, the genome DNA is degraded by an endonuclease-like action. Evidence is presented to show that the inducible inhibition of DNA degradation after x-irradiation (Pollard, E.C. and Randall, E.P. (1973) Radiat. Res. 55, 265) is probably caused by the inducible repair of DNA double-strand breaks.
Research Organization:
Yale Univ., New Haven, CT
DOE Contract Number:
AC02-76EV03571
OSTI ID:
6454622
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:6; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL CULTURES
CHEMISTRY
COBALT 60
COBALT ISOTOPES
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
EXTERNAL IRRADIATION
GAMMA RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RECOVERY
REPAIR
STRAND BREAKS
SYNTHESIS
ULTRAVIOLET RADIATION
YEARS LIVING RADIOISOTOPES