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recA (Srf) suppression of recF deficiency in the postreplication repair of UV-irradiated Escherichia coli K-12

Journal Article · · J. Bacteriol.; (United States)
OSTI ID:6829136
;

The mechanism by which recA (Srf) mutations (recA2020 and recA801) suppress the deficiency in postreplication repair shown by recF mutants of Escherichia coli was studied in UV-irradiated uvrB and uvrA recB recC sbcB cells. The recA (Srf) mutations partially suppressed the UV radiation sensitivity of uvrB recF, uvrB recF recB, and uvrA recB recC sbcB recF cells, and they partially restored the ability of uvrB recF and uvrA recB recC sbcB recF cells to repair DNA daughter-strand gaps. In addition, the recA (Srf) mutations suppressed the recF deficiency in the repair of DNA double-strand breaks in UV-irradiated uvrA recB recC sbcB recF cells. The recA2020 and recA801 mutations do no appear to affect the synthesis of UV radiation-induced proteins, nor do they appear to produce an altered RecA protein, as detected by two-dimensional gel electrophoresis. These results are consistent with the suggestion (M.R. Volkert and M.A. Hartke) that the recA (Srf) mutations do not act by affecting the induction of SOS responses; rather, they allow the RecA protein to participate in the recF-dependent postreplication repair processes without the need of the RecF protein.

Research Organization:
Stanford Univ. School of Medicine, CA
OSTI ID:
6829136
Journal Information:
J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 168:2; ISSN JOBAA
Country of Publication:
United States
Language:
English

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Related Subjects

550400 -- Genetics
560130* -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE REGULATION
MICROORGANISMS
MUTANTS
MUTATIONS
NUCLEIC ACID REPLICATION
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION