Different effects of recJ and recN mutations on the postreplication repair of UV-damaged DNA in Escherichia coli K-12

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · J. Bacteriol.; (United States)

OSTI ID:6862109

Wang, T C.V.; Smith, K C

Two mutations known to affect recombination in a recB recC sbcBC strain, recJ284::Tn10 and recN262, were examined for their effects on the postreplication repair of UV-damaged DNA. The recJ mutation did not affect the UV radiation sensitivity of uvrB and uvrB recF cells, but it increased the sensitivity of uvrB recN (approx. 3-fold) and uvrB recB (approx. 8-fold) cells. On the other hand, the recN mutation did not affect the UV sensitivity of uvrB recB cells, but it increased the sensitivity of uvrB (approx. 1.5-fold) and uvrB recF (approx. 4-fold) cells. DNA repair studies indicated that the recN mutation produced a partial deficiency in the postreplication repair of DNA double-strand breaks that arise from unrepaired daughter strand gaps, while the recJ mutation produced a deficiency in the repair of daughter strand gaps in uvrB recB cells (but not in uvrB cells) and a deficiency in the repair of both daughter strand gaps and double-strand breaks in uvrA recB recC sbcBC cells. Together, these results indicate that the recJ and recN genes are involved in different aspects of postreplication repair.

• Research Organization: Stanford Univ. School of Medicine, CA (USA)
• OSTI ID: 6862109
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 170:6; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560130* -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE MUTATIONS
GENE RECOMBINATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
KINETICS
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
REACTION KINETICS
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION