Relative rates of repair of single-strand breaks and postirradiation DNA degradation in normal and induced cells of Escherichia coli. [uv radiation]
Journal Article
·
· Biophys. J.; (United States)
Labeled DNA from irradiated Escherichia coli cells was studied on an alkaline sucrose gradient without acid precipitation of the DNA. This enabled the observation of both DNA repair and DNA degradation. The use of a predose of ultraviolet light (uv) caused induction of an inhibitor of postirradiation DNA degradation in le lambda/sup +/ strains. The effect of this induction on both the repair of single-strand breaks and DNA degradation was followed in strains WU3610 (uvr/sup +/) and WU3610-89 (uvr/sup -/). The repair process was more rapid than the degradation, and when degradation was inhibited more repair was apparent. Cells that were lex/sup -/ (B/sub s-1/ and AB2474) could not be induced for inhibition of degradation. Nevertheless, by observation at short times repair could be seen clearly. This repaired DNA was degraded, suggesting that the signal for DNA degradation is not a single-strand break.
- Research Organization:
- Pennsylvania State Univ., University Park
- OSTI ID:
- 6203816
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 24:2; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light
Basic aspects of radiation action on microorganisms. Progress report, April 1, 1976--June 30, 1977. [Escherichia coli, Pseudomonas, lambda phage, gamma and uv radiation]
Yield and repair of x-ray-induced single-strand breaks in the DNA of Escherichia coli K-12 cells
Journal Article
·
Mon Jun 01 00:00:00 EDT 1981
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6454622
Basic aspects of radiation action on microorganisms. Progress report, April 1, 1976--June 30, 1977. [Escherichia coli, Pseudomonas, lambda phage, gamma and uv radiation]
Technical Report
·
Fri Jul 01 00:00:00 EDT 1977
·
OSTI ID:7294542
Yield and repair of x-ray-induced single-strand breaks in the DNA of Escherichia coli K-12 cells
Journal Article
·
Fri Oct 01 00:00:00 EDT 1976
· Radiat. Res.; (United States)
·
OSTI ID:7330249
Related Subjects
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL VARIABILITY
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENETIC VARIABILITY
INHIBITION
IRRADIATION
MICROORGANISMS
NUCLEIC ACID DENATURATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL VARIABILITY
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENETIC VARIABILITY
INHIBITION
IRRADIATION
MICROORGANISMS
NUCLEIC ACID DENATURATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION