Yield and repair of x-ray-induced single-strand breaks in the DNA of Escherichia coli K-12 cells
Journal Article
·
· Radiat. Res.; (United States)
The yield of single-strand breaks present in chromosomal DNA of Escherichia coli cells after aerobic x irradiation was determined using alkaline sucrose gradient techniques. Experimental conditions were used that avoided a centrifugation speed dependence effect on DNA sedimentation, and allowed either minimal or complete enzymatic repair to occur. The results confirm the qualitative conclusions made in earlier reports from this laboratory, but indicate that the extent of DNA strand breakage was previously underestimated by factors of 3.4 to 7.6, depending on the strain and repair conditions used. Technical and interpretive difficulties in the measurement of DNA single-strand breakage using alkaline sucrose gradient techniques are discussed briefly. In the present experiments, the initial yield of DNA single-strand breaks was found to be 32.4/genome krad/sup -1/ (9.0 eV/break).
- Research Organization:
- Stanford Univ., CA
- OSTI ID:
- 7330249
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 68:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
IONIZING RADIATIONS
IRRADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
STRAND BREAKS
X RADIATION
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
IONIZING RADIATIONS
IRRADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
STRAND BREAKS
X RADIATION