CHO cell repair of single-strand and double-strand DNA breaks induced by $gamma$- and $alpha$-radiations

Conference · Wed Jan 01 04:00:00 EST 1975

OSTI ID:4120227

Cole, A; Shonka, F; Corry, P; Cooper, W G; Hanawalt, P C ^[1]

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1. ed.

Neutral and alkaline sucrose gradient sedimentation analysis was used to measure double- and single-strand breaks in the DNA of Chinese hamster ovary (CHO) cells exposed to either $gamma$- or $alpha$-radiation. After irradiation, cells were incubated for 15--180 min to test the ability of the cell to rejoin the DNA breaks. Essentially complete rejoining was observed for single-strand breaks inducedby $gamma$- or $alpha$-doses below 20 krad and for double-strand breaks induced by $gamma$ doses below 60 krad. Approximately 80 percent rejoining was observed for double-strand breaks induced by $alpha$ doses below 40 krad. At higher doses, the repair system appeared to saturate in such a way that essentially no additional breaks were rejoined. (auth)

• Research Organization: Anderson Hospital and Tumor Inst., Houston, TX
• NSA Number: NSA-33-012236
• OSTI ID: 4120227
• Country of Publication: United States
• Language: English

Related Subjects

*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
*DNA-- STRAND BREAKS
*STRAND BREAKS-- RADIOINDUCTION
560121* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Cells--External Source
ALPHA PARTICLES
GAMMA RADIATION
HAMSTERS
N48210* --Life Sciences--Radiation Effects on Cells-- External Source