Natural killer activity in the rat. IV. Distribution of large granular lymphocytes (LGL) following intravenous and intraperitoneal transfer

Journal Article · Sun Jul 01 04:00:00 EDT 1984 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(84)90392-7· OSTI ID:6449514

Reynolds, C W; Denn, A C; Barlozzari, T; Wiltrout, R H; Reichardt, D A; Herberman, R B

Highly enriched populations of rat large granular lymphocytes (LGL) and T lymphocytes were prepared on discontinuous density gradients of Percoll, labeled with either /sup 111/In-oxine or /sup 51/Cr and injected either intravenously (iv) or intraperitoneally (ip) into normal syngeneic recipients. Following iv inoculation of labeled LGL or T cells into normal recipients, a large proportion of radioactivity (18 to 33%) was recovered within minutes in the lungs. By 2 to 4 hr following transfer, significantly more LGL (13.5%) than T cells (6.4%) remained in the lungs. This difference persisted through 48 hr (5.4 vs 0.8%). Decreasing levels of radioactivity in the lungs were accompanied by corresponding increases in counts in the spleen and liver. At early time points, a significantly higher proportion of T cells was found to distribute to the spleen, while labeled LGL persisted for longer periods in the blood as well as in the lungs. Following ip inoculation into normal recipients, there was a slow clearance of radiolabeled LGL and T cells from the peritoneal cavity, with less than 20% of the radiolabel found in peripheral organs by 24 hr. These results demonstrate a distribution pattern for LGL and T cells that resembles the previously reported proportions of these cells in various organs. In addition, these studies provide a firm basis for the formulation of further experiments to examine the usefulness of adoptive immunotherapy with LGL or immune T cells.

• Research Organization: National Cancer Inst., MD
• OSTI ID: 6449514
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 86:2; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CLEARANCE
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
EXCRETION
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAPERITONEAL INJECTION
INTRAVENOUS INJECTION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LIVER
LUNG CLEARANCE
LUNGS
LYMPHOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXINE
PYRIDINES
QUINOLINES
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SPLEEN
TISSUE DISTRIBUTION
VERTEBRATES