Natural killer cell activity in the rat. V. Circulation patterns and tissue localization of peripheral blood large granular lymphocytes (LGL)

Conference · Tue Apr 15 04:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:6845991

Rolstad, B; Herberman, R B; Reynolds, C W

Large granular lymphocytes (LGL) and T cells were separated from blood by centrifugation on discontinuous gradients of Percoll, were labeled with (/sup 3/H)uridine or (/sup 111/In)oxine, and were injected i.v. into syngeneic euthymic or athymic nude rats. The tissue distribution of these labeled cells was monitored for up to 24 hr after transfer by scintillation counting of tissue homogenates and autoradiography of tissue sections. In normal euthymic rats, the main sites of LGL localization were the alveolar walls of the lungs and spleen red pulp; however, they were not detectable in the major traffic areas of T lymphocyte recirculation, the spleen white pulp, and lymph nodes. Furthermore, the density of labeled LGL was very low in the small intestine, thymus, kidney, and liver, although on a per-organ basis, about 10% of the injected radioactivity was found in the liver by 24 hr post-injection. When /sup 111/In-labeled LGL were injected i.v. into rats with an indwelling thoracic duct cannula, they completely failed to enter the thoracic duct lymphocyte (TDL) population over an observation period of 6 days. These findings define the areas within the lungs and spleen in which blood LGL normally localize, and clearly demonstrate that LGL do not normally recirculate between blood and lymph.

• Research Organization: National Institute of Health, Frederick, MD
• OSTI ID: 6845991
• Conference Information: Journal Name: J. Immunol.; (United States) Journal Volume: 136:8
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD CIRCULATION
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DYNAMIC FUNCTION STUDIES
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LIVER
LUNGS
LYMPH NODES
LYMPH VESSELS
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
SOMATIC CELLS
SPLEEN
THYMUS
TISSUE DISTRIBUTION
TRITIUM
URACILS
URIDINE
VERTEBRATES
YEARS LIVING RADIOISOTOPES