In vivo localization of cloned IL-2-dependent T cells

Journal Article · Tue Feb 15 04:00:00 EST 1983 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(83)90349-0· OSTI ID:5764916

Carroll, A M; Palladino, M A; Oettgen, H; De Sousa, M

The quantitative organ distribution and tissue microenvironment positioning of radioisotopically labeled cloned T cells were characterized. Intravenous (iv) injection of 51chromium (/sup 51/Cr)-labeled, long-term cultured cloned T-helper cells and cells from several cloned cytolytic T-lymphocyte lines (CTLL) resulted in poor localization of these cells in recipient lymphoid tissues, similar to results reported for activated lymphoblastoid cells. Simultaneous administration of interleukin 2 (IL-2) with labeled cells resulted in enhanced recovery from recipient spleen. By the intraperitoneal (ip) injection route, overall percentage recovery of injected radioactivity was lower than by the iv route, but significant localization to lymph nodes occurred. Examination of autoradiographs of tissue sections from recipients of (/sup 3/H)adenosine-labeled cells showed most label associated with intact, isolated cells in the liver, lungs, spleen, and small intestine. By 24 hr after iv injection, labeled cells in spleen sections were distributed to both nonlymphoid and T- and B-lymphoid areas. These findings suggest that poor localization of these cells to recipient lymphoid tissue is due both to intrinsic characteristics of cultured lymphocytes and to the possible reduced viability of IL-2-dependent cells in vivo.

• Research Organization: Department of Cell Ecology, Memorial Sloan-Kettering Cancer Center, New York, NY
• OSTI ID: 5764916
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 76:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL CULTURES
CHROMIUM 51
CHROMIUM ISOTOPES
CLONE CELLS
CONNECTIVE TISSUE CELLS
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRANSPLANTS
VERTEBRATES