In vivo distribution and tissue localization of highly purified rat lymphokine-activated killer (LAK) cells

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(88)90172-4· OSTI ID:7071236

Al Maghazachi, A; Herberman, R B; Vujanovic, N L; Hiserodt, J C

A highly purified population of effector lymphokine-activated killer (LAK) cells was generated by culturing nylon-wool column nonadherent rat splenocytes in the presence of interleukin 2 (IL-2), and the cells which became adherent to the plastic flasks were separated and maintained in culture for a total of 5 days. More than 95% of these cells had the morphology of large granular lymphocytes (LGL), expressed surface phenotypes characteristic of rat natural killer (NK) cells, and were able to kill NK-sensitive and NK-resistant tumor target cells. 51Cr-labeled purified A-LAK cells injected intravenously into syngeneic F344 rats localized primarily in the lungs 2 hr after injection but then redistributed to the liver and the spleen by 24 hr after injection. The effects of various immunological manipulations on the distribution pattern of the isolated LAK cells were evaluated. Treatment of the host with 500 rad total body X-irradiation 24 hr before cell injection resulted in an early uptake of LAK cells into the liver and the spleen, whereas treatment with cyclophosphamide 1 day before cell injection, resulted in an early uptake of LAK cells into the liver but not into the spleen. Treatment of the recipient rats with up to 120,000 units recombinant interleukin-2 intraperitoneally did not result in the accumulation of LAK cells at the site of IL-2 injection, nor did it result in a modulation of the overall distribution pattern or total recovery of radiolabeled LAK cells. Rather, the administration of IL-2 was necessary to maintain the cytotoxic activity of the injected LAK cells isolated from the liver and spleen.

• Research Organization: Pittsburgh Cancer Institute, PA (USA)
• OSTI ID: 7071236
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 115:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

551001 -- Physiological Systems-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
EXTERNAL IRRADIATION
FUNCTIONS
GLANDS
GROWTH FACTORS
IMMUNITY
IMMUNOSUPPRESSION
IN VIVO
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAPERITONEAL INJECTION
INTRAVENOUS INJECTION
IONIZING RADIATIONS
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIVER
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHENOTYPE
PROTEINS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION