Interleukin-2 (rIL-2)-induced lymphokine-activated killer (LAK) cells and their precursors express the VGO1 antigen

Journal Article · Sat Jul 01 04:00:00 EDT 1989 · Journal of Clinical Immunology; (USA)

DOI:https://doi.org/10.1007/BF00918668· OSTI ID:5364599

Denegri, J F; Peterson, J; Tilley, P ^[1]

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1. Vancouver General Hospital, B.C. (Canada)

Precursor and effector cells of recombinant interleukin-2 (r-IL-2)-induced lymphokine-activated killer (LAK) activity were investigated for their expression of VGO1. Peripheral blood lymphocytes (PBL) from normal donors were purified and separated in a FACS 420 into VGO1+- and VGO1- cell fractions before and after culture for 96 hr with 100 U/ml of r-IL-2. Their lytic activity against K 562 and Daudi cells was measured in a 51Cr release assay. The majority, if not all, of the LAK effector and precursor cells was VGO1+ lymphocytes. The expression of VGO1 by LAK precursor cells remained stable under the culture conditions used in our experiments. VGO1- lymphocytes cultured with r-IL-2 demonstrated neither LAK-induced activity nor expression of VGO1 antigen.

• OSTI ID: 5364599
• Journal Information: Journal of Clinical Immunology; (USA), Journal Name: Journal of Clinical Immunology; (USA) Vol. 9:4; ISSN 0271-9142; ISSN JCIMD
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FRACTIONATION
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
NUCLEI
PRECURSOR
PRIMATES
RADIOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
SOMATIC CELLS
STEM CELLS
TRACER TECHNIQUES
VERTEBRATES