Clonal analysis of the T lymphocytes involved in parent versus F1 graft-versus-host reaction
Journal Article
·
· Immunogenetics (N.Y.); (United States)
OSTI ID:6437490
A systemic graft-versus-host reaction (GVHR) leading to 50% mortality by day 20 was elicited by the injection of CBA (10(5)) or B10 (10(6)) parental T lymphocytes into irradiated (750 rad) and bone marrow protected (CBA X B10)F1 recipients. Between days 12 and 28 the spleens of the sick mice were analyzed by limiting dilution, performed with irradiated F1 cells and a source of interleukin-2 (IL-2), to determine the frequency of cells with an antihost proliferative or cytolytic activity and to derive T lymphocyte clones. The frequency of cells with antihost proliferative or cytolytic activity was approximately 10(-3) in either combination. In the CBA vs F1 GVHR, all eight clones isolated with anti-F1 activity were Lyt-2-, noncytolytic, mixed lymphocyte reaction (MLR) responders and IL-2 producers, three of which mapped to the Ab locus, while in the B10 anti-F1 combination, eight of the nine anti-F1 clones isolated were Lyt-2+, poor MLR responders and non-IL-2 producers, but cytolytic and mapping to Kk. These findings suggest a much higher frequency of T cells recognizing the A-locus antigens in the CBA than in the B10 strain.
- Research Organization:
- Department of Pathology, University of Geneva, Faculty of Medicine, Switzerland
- OSTI ID:
- 6437490
- Journal Information:
- Immunogenetics (N.Y.); (United States), Journal Name: Immunogenetics (N.Y.); (United States) Vol. 20:1; ISSN IMNGB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL CULTURES
CLONE CELLS
CONNECTIVE TISSUE CELLS
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
ORGANS
RODENTS
SOMATIC CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL CULTURES
CLONE CELLS
CONNECTIVE TISSUE CELLS
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
ORGANS
RODENTS
SOMATIC CELLS
TISSUES
TRANSPLANTS
VERTEBRATES