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Decreased superoxide anion radical production by rat alveolar macrophages following inhalation of ozone or nitrogen dioxide

Journal Article · · Life Sci.; (United States)
; ;

In vivo exposure of rats to ozone or nitrogen dioxide results in a dose-dependent increase in superoxide anion radical production (O/sub 2//sup -/.) by alveolar macrophages isolated from the exposed animals. When alveolar macrophages from ozone-exposed animals were stimulated with phorbol myristate acetate (PMA, a non-phagocytic stimulus of O/sub 2//sup -/. production) the decrease in O/sub 2//sup -/. production ranged from 85.9% of control at 3.2 ppm-hrs ozone to 7% of control at 10.5 ppm-hrs. In a similar fashion, O/sub 2//sup -/. production by PMA-stimulated macrophages from NO/sub 2/-exposed rats ranged from 78% of control at 18.3 ppm-hrs NO/sub 2/ down to 14.5% of control at 51 ppm-hrs. Since the viability of the alveolar macrophages obtained from ozone or nitrogen dioxide exposed animals was 88% or better in all cases as judged by both Trypan blue exclusion and lactate dehydrogenase release, the decreased ability of these cells to produce superoxide anion radical cannot be attributed to a pollutant effect on cell viability. This diminution in superoxide anion radical production by alveolar macrophages from the pollutant-exposed animals might account, in part, for the ability of these 2 air pollutants to potentiate bacterial infections in laboratory animals.

Research Organization:
Rutgers Medical School, Piscataway, NJ
OSTI ID:
6441865
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 28:20; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
551000 -- Physiological Systems
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANIONS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CHALCOGENIDES
CHARGED PARTICLES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
HEMIACETAL DEHYDROGENASES
IN VIVO
INFECTIVITY
INHALATION
INTAKE
IONS
KINETICS
LACTATE DEHYDROGENASE
LUNGS
MACROPHAGES
MAMMALS
METABOLISM
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OZONE
PHAGOCYTES
QUANTITY RATIO
RADICALS
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
SOMATIC CELLS
SUPEROXIDE RADICALS
VERTEBRATES
VIABILITY