Exposure of surfactant protein A to ozone in vitro and in vivo impairs its interactions with alveolar cells

Journal Article · Wed Jan 01 04:00:00 EST 1992 · American Journal of Physiology; (United States)

OSTI ID:5905622

Oosting, R S; Van Iwaarden, J F; Van Bree, L; Verhoef, J; Van Golde, L M; Haagsman, H P ^[1]

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1. Laboratory of Veterinary Biochemistry, Utrecht University (Netherlands)

This study focused on the question of whether exposure of surfactant protein A (SP-A) to ozone affected properties of this protein that may be involved in regulating alveolar type II cell and alveolar macrophage functions. In vitro exposure of human or canine SP-A to ozone reduced the ability of this protein to inhibit phorbol-ester induced secretion of (3H)phosphatidylcholine by alveolar type II cells in culture. Ozone-exposed human SP-A showed a decreased ability to enhance phagocytosis of herpes simplex virus and to stimulate superoxide anion production by alveolar macrophages. Experiments with elastase showed that ozone-exposed canine SP-A was more susceptible to proteolysis. A conformational change of the protein could underlie this phenomenon. Surfactant isolated from ozone-exposed rats (0.4 ppm ozone for 12 h) was also less able to stimulate superoxide anion production by alveolar macrophages than surfactant from control rats, which suggested that SP-A in vivo was also susceptible to ozone. The results of this study suggest that SP-A-alveolar cell interactions can be inhibited by ozone exposure, which may contribute to the toxicity of ozone in the lungs.

• OSTI ID: 5905622
• Journal Information: American Journal of Physiology; (United States), Journal Name: American Journal of Physiology; (United States) Vol. 262:1 Pt 1; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZO COMPOUNDS
AZO DYES
BIOLOGICAL EFFECTS
BODY
CARCINOGENS
CHEMICAL PROPERTIES
CONNECTIVE TISSUE CELLS
DOGS
DYES
ESTERS
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
LAVAGE
LIPIDS
LUNGS
MACROPHAGES
MAMMALS
MAN
NAPHTHOLS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OZONE
PHAGOCYTES
PHAGOCYTOSIS
PHENOLS
PHORBOL ESTERS
PHOSPHOLIPIDS
PRIMATES
PROTEINS
RATS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
SOMATIC CELLS
SULFONIC ACIDS
TOXICITY
TRYPAN BLUE
VERTEBRATES