Effect of ozone on alveolar macrophages and their microenvironment
Pulmonary alveolar macrophages (PAM), lung cells which inactivate bacteria, and, lung lavage fluid, were investigated following in vivo exposure to O/sub 3/. PAM isolated from Sprague-Dawley female rats exposed to 1.4 ppm O/sub 3/ for 3 hours exhibited a decrease in PAM superoxide anion radical (O/sub 2/) production (as measured by the superoxide dismutase inhibitable reduction of ferricytochrone c), phagocytosis (as measured by latex bead ingestion), membrane lipid fluidity (as measured by fluorescence polarization), and number of available sulfhydryl groups on the PAM plasma membrane (as measured by reaction between carboxypyridine disulfide and plasma membrane sulfhydryl groups). Incubation of control rat PAM with lung lavage fluid (LLF) from rats exposed to 3.0 ppm O/sub 3/ for 3 hours exhibited decreases in the same parameters, as compared to PAM incubated with LLF from control rats.
- Research Organization:
- Rutgers--the State Univ., New Brunswick, NJ (USA)
- OSTI ID:
- 6895204
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOSYNTHESIS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CONNECTIVE TISSUE CELLS
ENZYMES
LAVAGE
LUNGS
MACROPHAGES
MAMMALS
MEMBRANES
ORGANS
OXIDOREDUCTASES
OZONE
PHAGOCYTES
RADICALS
RATS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
SOMATIC CELLS
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
SYNTHESIS
TOXICITY
VERTEBRATES