DNA repair in V-79 cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene
Earlier experiments on human cells showed that N-acetoxy-2-acetylaminofluorene mimics ultraviolet radiation in biological and repair characteristics and that the amount of repair from a combined treatment was additive. Chinese hamster V-79 cells are less proficient than human cells in excision repair of pyrimidine dimers resulting from irradiation. We therefore investigated the combined effects of both agents on repair in V-79 cells to see whether they follow the same pattern as in human cells. They did not. Measurements of unscheduled DNA synthesis and the photolysis of DNA repaired in the presence of bromodeoxyuridine gave information about repair due to both agents, and the use of an endonuclease in an extract of Micrococcus luteus allowed us to measure repair of only ultraviolet damage in the presence of N-acetoxy-2-acetylaminofluorene damage. Each technique indicated that the amount of repair from a combined treatment was less than additive and in some cases less than that due to either agent. We conclude that V-79 cells are different from human fibroblasts in the excision repair of both ultraviolet and N-acetoxy-2-acetylaminofluorene damage and suggest that both kinds of damages inhibit repair of damage due to the other agent.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 6437408
- Journal Information:
- Cancer Res.; (United States), Vol. 37
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
DNA
BIOLOGICAL REPAIR
RADIOMIMETIC DRUGS
RADIOSENSITIVITY EFFECTS
BUDR
HAMSTERS
MICROCOCCUS LUTEUS
ULTRAVIOLET RADIATION
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BROMOURACILS
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
MICROCOCCUS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
URACILS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)