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Different rate-limiting steps in excision repair of ultraviolet- and N-acetoxy-2-acetylaminofluorene-damaged DNA in normal human fibroblasts

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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In normal human cells the amount of excision of ultraviolet damage to DNA saturates at high doses. In these cells some chemicals mimic ultraviolet damage as far as their biological and repair characteristics are concerned. One of these chemicals is N-acetoxy-2-acetylaminofluorene. We determined whether the limited repair capacity for ultraviolet damage was affected by treatment with N-acetoxy-2-acetylaminofluorene.To measure repair we determined unscheduled DNA synthesis and the number of sites sensitive to an ultraviolet endonuclease in an assay using an extract of Micrococcus luteus. The nuclease does not act on DNA treated with the chemical. The amount of unscheduled DNA synthesis due to a combined chemical and ultraviolet treatment was the sum of those observed from the separate treatments, even at saturation doses. The combined treatment did not affect the removal of nuclease-sensitive sites. We conclude that there are different rate-limiting steps in excision repair of the ultraviolet and the chemical damage and suggest a model involving a complex of enzymes to explain the data.
Research Organization:
Brookhaven National Lab., Upton, NY
OSTI ID:
7142805
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 74:4; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
FIBROBLASTS
IRRADIATION
MAMMALS
MAN
MUTAGENS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHOTRANSFERASES
PRIMATES
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES