Kinetics of DNA repair in ultraviolet-irradiated and N-acetoxy-2-acetylaminofluorene-treated mamalian cells. [Comparison of repair kinetics in cultured human fibroblasts and Chinese hamster cells (V-79)]

Journal Article · Fri Dec 01 04:00:00 EST 1978 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(78)85412-5· OSTI ID:6469930

Ahmed, F E; Setlow, R B

Repair kinetics after saturating doses of ultraviolet radiation (uv), N-acetoxy-2-acetylaminofluorene (AAAF), and combinations of both agents were studied in human fibroblasts proficient and deficient in excision repair, and in Chinese hamster cells (V-79) deficient in excision repair. Three techniques were used: unscheduled DNA synthesis, photolysis of DNA repaired in the presence of bromodeoxyuridine (BrdUrd), and measurements of sites sensitive to a uv-endonuclease. The repair rate appears to be approximately constant during the first few hours after treatment. Later there is a decrease with time; the magnitude of the decrease depends on the cell line. Our data show that the decrease in repair observed in repair-deficient cells treated with combinations of both agents as compared to separate treatments is due neither to the cytotoxic effects of the agents used, nor to a shutoff of the repair system by the high concentrations of AAAF employed.

• Research Organization: Brookhaven National Lab., Upton NY
• OSTI ID: 6469930
• Journal Information: Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 24:3; ISSN BIOJA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400500 -- Photochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CARCINOGENS
CELL CULTURES
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DECOMPOSITION
DNA
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IRRADIATION
KINETICS
MAMMALS
MAN
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PRIMATES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
REACTION KINETICS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES