Kinetics of DNA repair in ultraviolet-irradiated and N-acetoxy-2-acetylaminofluorene-treated mamalian cells. [Comparison of repair kinetics in cultured human fibroblasts and Chinese hamster cells (V-79)]
Repair kinetics after saturating doses of ultraviolet radiation (uv), N-acetoxy-2-acetylaminofluorene (AAAF), and combinations of both agents were studied in human fibroblasts proficient and deficient in excision repair, and in Chinese hamster cells (V-79) deficient in excision repair. Three techniques were used: unscheduled DNA synthesis, photolysis of DNA repaired in the presence of bromodeoxyuridine (BrdUrd), and measurements of sites sensitive to a uv-endonuclease. The repair rate appears to be approximately constant during the first few hours after treatment. Later there is a decrease with time; the magnitude of the decrease depends on the cell line. Our data show that the decrease in repair observed in repair-deficient cells treated with combinations of both agents as compared to separate treatments is due neither to the cytotoxic effects of the agents used, nor to a shutoff of the repair system by the high concentrations of AAAF employed.
- Research Organization:
- Brookhaven National Lab., Upton NY
- OSTI ID:
- 6469930
- Journal Information:
- Biophys. J.; (United States), Vol. 24:3
- Country of Publication:
- United States
- Language:
- English
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Excision repair in mammalian cells. [uv radiation, N-acetoxy-2-acetylaminofluorene]
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BIOLOGICAL REPAIR
BIOCHEMICAL REACTION KINETICS
COMPARATIVE EVALUATIONS
CARCINOGENS
RADIOSENSITIVITY EFFECTS
DNA
STRAND BREAKS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BUDR
CELL CULTURES
FIBROBLASTS
HAMSTERS
IRRADIATION
MAN
ORGANIC FLUORINE COMPOUNDS
PHOTOLYSIS
ULTRAVIOLET RADIATION
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL RECOVERY
BROMOURACILS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DECOMPOSITION
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PRIMATES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
URACILS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
400500 - Photochemistry