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Defective and enhanced postreplication repair in classical and variant xeroderma pigmentosum cells treated with N-acetoxy-2-acetylaminofluorene

Journal Article · · Cancer Res.; (United States)
OSTI ID:5544247
;
Xeroderma pigmentosum (XP) cells proficient in the excision repair of pyrimidine dimers (XP variants) were also found to be proficient in the excision repair of N-2-acetoxyacetylaminofluorene (AAAF)-induced lesions in their DNA, as assayed by the photolysis of 5-bromodeoxyuridine incorporated during repair. However, the time in which the small segments of newly synthesized DNA, made immediately after treatment of cells with AAAF, were joined together to form DNA of parental size by a process called postreplication repair was long in the XP variant and classical cells. Although increasing doses of AAAF increased the time for making daughter DNA of parental size for variant and classical XP cells, AAAF did not appear to affect this process in normal human cells. Treatment of variant and classical XP cells with a relatively small dose (2.5 micron) of AAAF or 2.5 J/m/sup 2/ of uv radiation several h before a 2- to 3-fold-larger dose decreased the time for the pulse-labeled DNA to appear as parental size.
Research Organization:
Brookhaven National Lab., Upton, NY
OSTI ID:
5544247
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 38:4; ISSN CNREA
Country of Publication:
United States
Language:
English

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Defective and enhanced postreplication repair in classical and variant xeroderma pigmentosum cells treated with N-acetoxy-2-acetylaminofluorene
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Related Subjects

550300 -- Cytology
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
COMPARATIVE EVALUATIONS
CONDENSED AROMATICS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
TIME DEPENDENCE
TOXICITY
ULTRAVIOLET RADIATION
XP CELLS