Defective and enhanced postreplication repair in classical and variant xeroderma pigmentosum cells treated with N-acetoxy-2-acetylaminofluorene

Journal Article · Sat Apr 01 04:00:00 EST 1978 · Cancer Res.; (United States)

OSTI ID:6851027

D'Ambrosio, S M; Setlow, R B

Xeroderma pigmentosum (XP) cells proficient in the excision repair of pyrimidine dimers (XP variants) were also found to be proficient in the excision repair of N-2-acetoxyacetylaminofluorene (AAAF)-induced lesions in their DNA, as assayed by the photolysis of 5-bromodeoxyuridine incorporated during repair. However, the time in which the small segments of newly synthesized DNA, made immediately after treatment of cells with AAAF, were joined together to form DNA of parental size by a process called postreplication repair was long in the XP variant and classical cells. Although increasing doses of AAAF increased the time for making daughter DNA of parental size for variant and classical XP cells, AAAF did not appear to affect this process in normal human cells. Treatment of variant and classical XP cells with a relatively small dose (2.5 ..mu..M) of AAAF or 2.5 J/sq m of uv radiation several hr before a 2- to 3-fold-larger dose decreased the time for the pulse-labeled DNA to appear as parental size.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 6851027
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 38:4; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550600 -- Medicine
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
AROMATICS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMINE COMPOUNDS
CARCINOGENS
CHEMICAL REACTIONS
DECOMPOSITION
DEOXYURIDINE
DIMERS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SKIN DISEASES
ULTRAVIOLET RADIATION
URACILS
XERODERMA PIGMENTOSUM
XP CELLS