DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene
Three techniques were used to examine excision repair in human cells treated with ultraviolet radiation, N-acetoxy-2-acetylaminofluorene, and a combination of the two. The three techniques gave similar results. Two types of human cells were used: (a) excision repair proficient (normal human fibroblasts and xeroderma pigmentosum variants); and (b) excision repair deficient (xeroderma pigmentosum, C, D, and E). Saturation doses were determined and used for combined treatments with both agents. We observed two patterns of repair: (a) in repair-proficient cells total repair was additive; and (b) in repair-deficient cells total repair was much less than additive (usually less than that observed for separate treatments) and N-acetocy-2-acetyl-aminofluorene inhibited excision of pyrimidine dimers. We conclude that, in the first group of cells, pathways for repair of ultraviolet radiation- and N-acetoxy-2-acetylaminofluorene-induced lesions are not identical and, in the second group of cells, there is an inhibitory effect exerted by major or minor products of each agent on the repair enzyme(s) of the other.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 5775415
- Journal Information:
- Cancer Res.; (United States), Vol. 39
- Country of Publication:
- United States
- Language:
- English
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Defective and enhanced postreplication repair in classical and variant xeroderma pigmentosum cells treated with N-acetoxy-2-acetylaminofluorene
Defective and enhanced postreplication repair in classical and variant xeroderma pigmentosum cells treated with N-acetoxy-2-acetylaminofluorene
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
BIOLOGICAL REPAIR
XERODERMA PIGMENTOSUM
ACETYL RADICALS
ANIMAL CELLS
ENZYME INHIBITORS
FIBROBLASTS
FLUORENE
MAN
PYRIMIDINES
SYNERGISM
ULTRAVIOLET RADIATION
ACYL RADICALS
ANIMALS
AROMATICS
AZINES
BIOLOGICAL RECOVERY
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
RADIATIONS
RADICALS
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
VERTEBRATES
550200* - Biochemistry
550300 - Cytology
560400 - Other Environmental Pollutant Effects
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)