DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene

Journal Article · Thu Feb 01 04:00:00 EST 1979 · Cancer Res.; (United States)

OSTI ID:5775415

Ahmed, F E; Setlow, R B

Three techniques were used to examine excision repair in human cells treated with ultraviolet radiation, N-acetoxy-2-acetylaminofluorene, and a combination of the two. The three techniques gave similar results. Two types of human cells were used: (a) excision repair proficient (normal human fibroblasts and xeroderma pigmentosum variants); and (b) excision repair deficient (xeroderma pigmentosum, C, D, and E). Saturation doses were determined and used for combined treatments with both agents. We observed two patterns of repair: (a) in repair-proficient cells total repair was additive; and (b) in repair-deficient cells total repair was much less than additive (usually less than that observed for separate treatments) and N-acetocy-2-acetyl-aminofluorene inhibited excision of pyrimidine dimers. We conclude that, in the first group of cells, pathways for repair of ultraviolet radiation- and N-acetoxy-2-acetylaminofluorene-induced lesions are not identical and, in the second group of cells, there is an inhibitory effect exerted by major or minor products of each agent on the repair enzyme(s) of the other.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 5775415
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39; ISSN CNREA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYL RADICALS
ACYL RADICALS
ANIMAL CELLS
ANIMALS
AROMATICS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
FIBROBLASTS
FLUORENE
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
MAMMALS
MAN
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATIONS
RADICALS
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
SYNERGISM
ULTRAVIOLET RADIATION
VERTEBRATES
XERODERMA PIGMENTOSUM