Overlapping pathways for repair of damage from ultraviolet light and chemical carcinogens in human fibroblasts

Journal Article · Sun Jul 01 04:00:00 EDT 1979 · Cancer Res.; (United States)

OSTI ID:5435002

Brown, A J ^[1]; Fickel, T H; Cleaver, J E; Lohman, P H.M.; Wade, M H; Waters, R

---

1. Oak Ridge National Lab., TN

DNA excision repair was measured in cultured human fibroblasts after single or dual treatments with ultraviolet radiation, 4-nitroquinoline 1-oxide, or N-acetoxy-2-acetylaminofluorene. Three approaches were used to monitor repair: unscheduled DNA synthesis, measured by autoradiography; repair replication, measured by the incorporation of a density-labeled DNA precursor into repaired regions; and excision of ultraviolet endonuclease-sensitive sites. When a single repair-saturating dose of one of the three carcinogens was administered, little stimulation of unscheduled DNA synthesis or repair replication could be observed by additional treatment with one of the other carcinogens. In no instance was total additivity of repair observed. These observations were confirmed by showing that the excision of endonuclease-sensitive sites produced by ultraviolet damage (i.e., pyrimidine dimers) was inhibited by exposure to 4-nitroquinoline 1-oxide and N-acetoxy-2-acetylaminofluorene. The data indicate that the repair of lesions induced by these substances may have common rate-limiting steps, a conclusion previously indicated by the repair deviciency in xeroderma pigmentosum cells in which a single mutation eliminates the repair of damage caused by each of these agents.

• OSTI ID: 5435002
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CARCINOGENS
CELL CULTURES
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
DIMERS
DNA
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
INJURIES
MAMMALS
MAN
NITROGEN COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
SOMATIC CELLS
SYNTHESIS
TOXICITY
ULTRAVIOLET RADIATION
VERTEBRATES