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Aldolase A isoenzyme levels in serum and tissues of patients with liver diseases

Journal Article · · Gastroenterology; (United States)
OSTI ID:6430701
; ; ;
A radioimmunoassay specific for human aldolase A was used to measure human aldolase A levels in human tissue and serum of patients with various liver diseases. The method was a double-antibody technique using radio-iodinated purified aldolase A, chicken antibody to aldolase A, and rabbit antibody to chicken immunoglobulin G. Normal liver tissue contains only a small amount of aldolase A. In contrast, aldolase A predominates in liver cell carcinoma tissue. Aldolase A levels in the sera of normal subjects were 171 +/- 39 ng/ml (mean +/- 2 SD). In almost all of the nonmalignant liver diseases, the aldolase A levels remained less than 210 ng/ml. The serum aldolase A levels increased remarkable only in fulminant hepatitis. in contrast, 32 of 34 patients with liver cell carcinoma and all of 29 patients with metastatic liver carcinoma showed clearly increased serum aldolase A levels. More patients with primary liver cell carcinoma had increased serum aldolase A levels than elevations of serum alpha-fetoprotein. These results suggest that the determination of aldolase A by radioimmunoassay may be useful to differentiate malignant form nonmalignant liver diseases.
Research Organization:
Department of Medicine, Baylor College of Medicine, Houston, Texas
OSTI ID:
6430701
Journal Information:
Gastroenterology; (United States), Journal Name: Gastroenterology; (United States) Vol. 84:1; ISSN GASTA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDE-LYASES
ALDOLASE
BLOOD SERUM
BODY
CARBON-CARBON LYASES
CARCINOMAS
DIAGNOSIS
DIAGNOSTIC USES
DIGESTIVE SYSTEM
DIGESTIVE SYSTEM DISEASES
DISEASES
ENZYMES
GLANDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
LYASES
METASTASES
NEOPLASMS
ORGANS
PATHOLOGY
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
TRACER TECHNIQUES
USES