Radioimmunoassay of creatine kinase-B isoenzyme in human sera: results in patients with acute myocardial infarction

Journal Article · Fri Apr 01 04:00:00 EST 1977 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.74.4.1711· OSTI ID:7312161

Willerson, J T; Stone, M J; Ting, R; Mukherjee, A; Gomez-Sanchez, C E; Lewis, P; Hersh, L B

A radiommunoassay was developed to measure serum levels of the B isoenzyme of creatine kinase (ATP: creatine N-phosphotransferase, EC 2.7.3.2) (CPK) in order to evaluate the time course and frequency of MB isoenzyme elevation in patients with acute myocardial infarction. The method can identify as little as 0.2 ng of the B portion of the CPK-MB isoenzyme, does not significantly crossreact with CPK-MM isoenzyme, and is not affected by storage of serum at -20/sup 0/. CPK isoenzyme containing B subunits was detected in 48 out of 51 sera from normal adults; serum levels in these individuals ranged between 1.2 and 12.5 ng/ml(mean +- SEM was 2.7 +- 0.30 ng/ml). The mean serum level of CPK-B isoenzyme in a pool of sera obtained from 100 normal subjects was 2.9 +- 0.35 ng/ml; two patients with rhabdomyolysis that were studied had serum CPK-B isoenzyme levels of 2.5 and 3.5 ng/ml, respectively. In contrast, serum levels of the CPK-B isoenzyme were markedly elevated in sera from 18 patients with acute myocardial infarcts when obtained within 12 hr after hospital admission; the mean +- SEM concentration was 56 +- 7.8 ng/ml. We performed serial determinations on 14 patients with acute myocardial infarcts and demonstrated that maximal serum CPK-B levels occurred within the first 12 hr after admission and were lower thereafter. The serum concentration of B-containing CPK isoenzyme in 19 additional patients admitted with chest pain but without acute myocardial infarction was 3.4 +- 0.50 ng/ml. Thus, radioimmunoassay measurement of CPK-B isoenzyme appears to be a useful and sensitive test for the detection of acute myocardial infarcts in patients.

• Research Organization: Univ. of Texas, Dallas
• OSTI ID: 7312161
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 74:4; ISSN PNASA
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD SERUM
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MAN
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NUCLEI
ODD-EVEN NUCLEI
PATIENTS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES