Lung, aorta, and platelet metabolism of /sup 14/C-arachidonic acid in vitamin E deficient rats

Journal Article · Sun Aug 01 04:00:00 EDT 1982 · Prostaglandins; (United States)

OSTI ID:6422729

Valentovic, M A; Gairola, C; Lubawy, W C

/sup 14/C-arachidonic acid metabolism was determined in aortas, platelets, and perfused lungs from rats pair fed a basal diet supplemented with 0 or 100 ppm vitamin E for 11 weeks. Spontaneous erythrocyte hemolysis tests showed 92% and 8% hemolysis for the 0 and 100 ppm vitamin E groups, respectively. Elevated lung homogenate levels of malonaldehyde in the 0 ppm group confirmed its deficient vitamin E status. Aortas from the vitamin E deficient group synthesized 54% less prostacyclin than aortas from the supplemented group (p less than 0.05). Although thromboxane generation by platelets from the vitamin E deficient group exhibited a 37% increase, this difference was not statistically significant compared to the supplemented animals. Greater amounts of PGE2, PGF2 alpha, TXB2, and 6-keto-PGF1 alpha were obtained in albumin buffer perfusates from lungs of vitamin E deficient rats than in those from supplemented rats. Significant differences (p less than 0.05) were noticed, however, only for PGE2 and PGF2 alpha. These studies indicate that vitamin E quantitatively alters arachidonic acid metabolism in aortic and lung tissue but its effect on thromboxane synthesis by platelets is less marked.

• Research Organization: College of Pharmacy University of Kentucky Lexington
• OSTI ID: 6422729
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 24:2; ISSN PRGLB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AORTA
ARACHIDONIC ACID
ARTERIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DIET
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LUNGS
MAMMALS
MATERIALS
METABOLISM
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERFUSED TISSUES
PROSTAGLANDINS
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
TISSUES
VERTEBRATES