The metabolism of arachidonic acid in isolated perfused fetal and neonatal rabbit lungs

Journal Article · Sun May 01 00:00:00 EDT 1983 · Prostaglandins; (United States)

DOI:https://doi.org/10.1016/0090-6980(83)90117-X· OSTI ID:5551692

Simberg, N; Uotila, P

The developmental pattern of fetal and neonatal rabbit lungs to metabolize arachidonic acid (AA) to different cyclo-oxygenase products was studied in isolated rabbit lungs, which were perfused with Krebs bicarbonate buffer. /sup 14/C-AA (66 nmol) was injected into the pulmonary circulation and the nonrecirculating perfusion effluent was collected for four minutes. About ten per cent of the injected radioactivity was found in the 0-4 min perfusion effluent. The metabolites of AA in the effluent were analyzed by thin layer chromatography. The major metabolites of AA were PGE2 and its 15-keto-derivates, but also PGF2 alpha and its 15-keto-derivates, TXB2 and 6-keto-PGF1 alpha were found in the effluent. The most drastic developmental change was the increase in the amount of 15-keto-metabolites of PGE2 from late fetal period to the lungs of one day old rabbits (1.8 fold increase between birth and first postnatal day). Smaller changes were detected in the amounts of other cyclo-oxygenase products.

• Research Organization: Department of Physiology, University of Turku, Finland
• OSTI ID: 5551692
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 25:5; ISSN PRGLB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
FETUSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
NEONATES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROSTAGLANDINS
RABBITS
RESPIRATORY SYSTEM
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
VERTEBRATES