Pulmonary arachidonic acid metabolism following acute exposures to ozone and nitrogen dioxide

Journal Article · Fri Nov 30 23:00:00 EST 1990 · Journal of Toxicology and Environmental Health; (USA)

DOI:https://doi.org/10.1080/15287399009531456· OSTI ID:6267435

Schlesinger, R B; Driscoll, K E; Gunnison, A F; Zelikoff, J T ^[1]

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1. New York Univ. Medical Center, NY (USA)

Ozone (O{sub 3}) and nitrogen dioxide (NO{sub 2}) are common air pollutants, and exposure to these gases has been shown to affect pulmonary physiology, biochemistry, and structure. This study examined their ability to modulate arachidonic acid metabolites (eicosanoids) in the lungs. Rabbits were exposed for 2 h to O{sub 3} at 0.1, 0.3, or 1 ppm; NO{sub 2} at 1, 3, or 10 ppm; or to a mixture of 0.3 ppm O{sub 3} and 3 ppm NO{sub 2}. Groups of animals sacrificed either immediately or 24 h after each exposure underwent broncho-pulmonary lavage. Selected eicosanoids were assessed in lavage fluid by radioimmunoassay. Increases in prostaglandins E2 (PGE2) and F2 alpha (PGF2 alpha) were found immediately after exposure to 1 ppm O{sub 3}. Exposure to 10 ppm NO{sub 2} resulted in a depression of 6-keto-PGF1 alpha, while thromboxane B2 (TxB2) was elevated after exposure to 1 ppm NO{sub 2} and depressed following 3 and 10 ppm. The O{sub 3}/NO{sub 2} mixture resulted in synergistic increases in PGE2 and PGF2 alpha, with the response appearing to be driven by O{sub 3}. This study has demonstrated that acute exposure to either O{sub 3} or NO{sub 2} can alter pulmonary arachidonic acid metabolism and that the responses to these oxidants differ, both quantitatively and qualitatively.

• OSTI ID: 6267435
• Journal Information: Journal of Toxicology and Environmental Health; (USA), Journal Name: Journal of Toxicology and Environmental Health; (USA) Vol. 31:4; ISSN JTEHD; ISSN 0098-4108
• Country of Publication: United States
• Language: English

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
AIR POLLUTION
ANIMALS
ARACHIDONIC ACID
BIOASSAY
BODY
CARBOXYLIC ACIDS
CHALCOGENIDES
DIAGNOSTIC TECHNIQUES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LAVAGE
LUNGS
MAMMALS
METABOLISM
METABOLITES
MONOCARBOXYLIC ACIDS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
OZONE
POLLUTION
PROSTAGLANDINS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RESPIRATORY SYSTEM
SENSITIVITY
SYNERGISM
TOXICITY
TRACER TECHNIQUES
VERTEBRATES