Effects of hyperthermia and ionizing radiation in normal and ataxia telangiectasia human fibroblast lines

Journal Article · Sat Sep 01 04:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576336· OSTI ID:6411967

Mitchel, R E.J.; Chan, A; Smith, B P; Child, S D; Paterson, M C

The effects of 45/sup 0/C hyperthermia and ..gamma.. radiation have been studied in three normal human fibroblast lines (GM38, GM730, WI38) and compared to the effects in two lines derived from patients with the hereditary disease ataxia telangiectasia (AR3BI, AT5BI). All lines, both normal and ..gamma..-sensitive AT, showed a similar resistance to killing by heat alone, suggesting that the defect responsible for the increased radiation sensitivity in AT lines does not confer increased heat sensitivity. Shouldered survival curves were obtained in each case indicating the ability to accumulate sublethal heat damage. All normal and AT cell lines exhibited increased resistance to the lethal effects of heat in response to a thermal stress, indicating that the defect that causes radiosensitivity in AT cell lines does not prevent the induction of thermotolerance. It was hypothesized that in normal cells, this heat treatment inactivates the process which is already defective in AT lines, and that this process may be required for the proper rejoining of double-strand breaks produced during the repair of other radiation-induced lesions.

• Research Organization: Chalk River Nuclear Laboratories, Ontario
• OSTI ID: 6411967
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 99:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY TEMPERATURE
CELL KILLING
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
DISEASES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
GAMMA RADIATION
HYPERTHERMIA
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
SKIN DISEASES
SOMATIC CELLS
STRAND BREAKS
SURVIVAL CURVES
SYNERGISM
TELANGIECTASIS
TEMPERATURE EFFECTS
VASCULAR DISEASES
YEARS LIVING RADIOISOTOPES