Use of Aroclor 1254-induced rat liver homogenate in the assaying of promutagens in Chinese hamster ovary cells

Journal Article · Sat Dec 31 23:00:00 EST 1983 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860060407· OSTI ID:6411086

Li, A P

Seven promutagens belonging to two chemical classes - polycyclic aromatic hydrocarbons (PAHs) and alkyl nitrosamines - were studied in Chinese hamster ovary (CHO) cells. Findings of practical importance in the use of Aroclor 1254-induced rat liver homogenate (S9) in the CHO/hypoxanthine-guanine phosphoribosyl transferase (HGPRT) mutation assay were made. Novel findings are (1) the inclusion of CaCl/sub 2/ in the S9 cofactor mixture dramatically decreased the cytotoxicity of S9, and (2) different S9 optimum concentrations were observed for struturally similar promutagens. The inclusion of CaCl/sub 2/ in the S9 cofactor mixture and the testing of each chemical of unknown S9 requirement at several S9 concentrations are therefore recommended for assaying promutagens in the CHO/HGPRT mutation assay.

• Research Organization: Monsanto Co., St. Louis, MO
• OSTI ID: 6411086
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 6:4; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METAL COMPOUNDS
AMINES
ANIMAL CELLS
AROMATICS
BIOASSAY
BIOLOGICAL EFFECTS
CALCIUM CHLORIDES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CHLORIDES
CHLORINE COMPOUNDS
CHO CELLS
ENZYMES
GENETIC EFFECTS
GLYCOSYL TRANSFERASES
HALIDES
HALOGEN COMPOUNDS
HYDROCARBONS
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
METABOLIC ACTIVATION
MUTAGENESIS
MUTATION FREQUENCY
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSYL TRANSFERASES
POLYCYCLIC AROMATIC HYDROCARBONS
TRANSFERASES