Quantitative mutagenesis and mutagen screening with Chinese hamster ovary cells

Conference · Mon Dec 31 23:00:00 EST 1979

OSTI ID:5219970

Hsie, A. W.; San Sebastian, J. R.; Tan, E. L.

A summary is presented on the development of a specific gene mutation assay, the Chinese hamster ovary cells/hypoxanthine-guanine phosphoribosyl transferase (CHO/HGPRT) system, and the utilization of this system to study structure-activity relationship affecting cytotoxicity and gene mutation by various carcinogens. Then, preliminary development and validation of a Multiplex CHO System for the simultaneous determination of chromosome aberration, sister chromatid exchange in addition to cytotoxicity and gene mutation is presented. The potential use of a CHO/human cell hybrid system for measuring chromosomal deletion and loss is discussed.

• Research Organization: Tennessee Univ., Oak Ridge (USA). Graduate School of Biomedical Sciences; Oak Ridge National Lab., TN (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5219970
• Report Number(s): CONF-800485-1
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
CARCINOGENS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
DATA
EXPERIMENTAL DATA
GENE MUTATIONS
HAMSTERS
INFORMATION
MAMMALS
MUTAGEN SCREENING
MUTATIONS
NUMERICAL DATA
RODENTS
SCREENING
SISTER CHROMATID EXCHANGES
TOXICITY
VERTEBRATES