Radiation response of Chinese hamster cells after elevation of intracellular glutathione levels

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(84)90326-2· OSTI ID:6408280

Russo, A; Mitchell, J B

Cellular glutathione (GSH) levels were modulated by either inhibition of GSH synthesis by buthionine sulfoximine (BSO) or elevation of GSH by treatment with 2-oxo-thiazolidine-4-carboxylate (OTZ), cobaltous chloride, or cysteamine. Using these agents, x ray survival in air was assessed as a function of cellular GSH levels. Depletion of GSH by GSO resulted in slight sensitization of the aerated curve. However, elevation of GSH by as much as 200 to 300% of controls provided no radioprotection in air. These data are discussed in the context of the role of GSH and GSH peroxidase in the detoxification of peroxides produced by x rays.

• Research Organization: National Cancer Inst., Bethesda, MD
• OSTI ID: 6408280
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 10:8; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CELL KILLING
CHLORIDES
CHLORINE COMPOUNDS
COBALT CHLORIDES
COBALT COMPOUNDS
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERS
FIBROBLASTS
GLUTATHIONE
HALIDES
HALOGEN COMPOUNDS
HAMSTERS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMINES
IONIZING RADIATIONS
MAMMALS
MEA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PORPHYRINS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
SYNTHESIS
THIOLS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
X RADIATION