Modification of SR 2508 sensitization in hypoxic V79 cells by manipulation of glutathione levels

Journal Article · Mon May 01 04:00:00 EDT 1989 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(89)90310-6· OSTI ID:6074153

Phillips, T L; Mitchell, J B; DeGraff, W G; Russo, A; Albright, N; Rajpal, R

This series of experiments employed the hypoxic cell sensitizer SR 2508 in concentrations ranging from 0.1 to 10 mM and V-79 cells irradiated in air or made hypoxic in glass syringes, then irradiated with 15 MV X rays. Using a series of survival curves measured at the various concentrations, K curves relating sensitizer enhancement ratio (SER) to SR 2508 concentration were calculated with normal GSH levels or with depletion of GSH to 0% using 1 mM buthionine sulfoximine (BSO) or elevation to 200% of normal using 1 mM oxothiazolidine carboxylate (OTZ). Survival curves were fitted by computer, allowing calculation of standard errors for the SER values. The depletion of GSH by BSO sensitized hypoxic and aerated cells significantly and caused more than additive enhancement of SR 2508 sensitization in hypoxic cells. Elevation of GSH with OTZ protects cells irradiated in air or hypoxia and reduces the SER obtained with SR 2508. The results further support the importance of GSH levels in influencing sensitization by nitroimidazoles.

• OSTI ID: 6074153
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 16:5; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANOXIA
AZOLES
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
FIBROBLASTS
GLUTATHIONE
HAMSTERS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IONIZING RADIATIONS
LIPOTROPIC FACTORS
LUNGS
MAMMALS
METHIONINE
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXYGEN
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
SYNTHESIS
THIAZOLES
VERTEBRATES
X RADIATION