Thiols, thiol depletion, and thermosensitivity

Conference · Thu Sep 01 04:00:00 EDT 1983 · Radiat. Res.; (United States)

OSTI ID:5527633

Mitchell, J B; Russo, A

Hyperthermia sensitization or tolerance is subject to cellular events that may occur at membrane, nuclear, and cytoplasmic sites. We have studied the effects of elevated temperatures on the oxidative-reductive state of the cell by measuring and altering glutathione (GSH) concentrations. GSH plays a pivotal role in maintaining the overall cellular redox state and detoxification of peroxides. Continuous heating at 42.5/sup 0/C or acute exposure at 43/sup 0/C or 45.5/sup 0/C resulted in rapid elevations of cellular GSH to 120 to 200% of control values. Qualitatively, the more severe the heat exposure, the quicker the maximal GSH levels are attained. Ethanol, a compound that also induces thermal tolerance, likewise increases intracellular GSH concentrations. GSH depletion by two different modalities, diethylmaleate (DEM) and buthionine sulfoximine (BSO), results in thermal sensitization. It was demonstrated that once thermotolerance has been induced, depletion of GSH has minimal effects on subsequent heating and thermotolerance. Heat shock protein (HSP) synthesis is lessened by treatment with buthionine sulfoximine; the extent of the decrease in HSP production correlates with the decrease in thermotolerance. A proposed mechanism of peroxide-induced cell damage is suggested by exogenous thiols, as well as an involvement of GSH in the initial aspects of thermotolerance induction.

• Research Organization: National Cancer Inst., Bethesda, MD
• OSTI ID: 5527633
• Conference Information: Journal Name: Radiat. Res.; (United States) Journal Volume: 95:3
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOSYNTHESIS
BODY TEMPERATURE
CHEMISTRY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FIBROBLASTS
GLUTATHIONE
HAMSTERS
HYPERTHERMIA
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDES
PEROXIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SOMATIC CELLS
SULFOXIDES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TEMPERATURE EFFECTS
THIOLS
VERTEBRATES