Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Glutathione depletion, radiosensitization, and misonidazole potentiation in hypoxic Chinese hamster ovary cells by buthionine sulfoximine

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576244· OSTI ID:7054862
; ; ; ;

Buthionine sulfoximine (BSO) inhibits the synthesis of glutathione (GSH), the major nonprotein sulfhydryl (NPSH) present in most mammalian cells. BSO exposures used in these experiments were not cytotoxic with the one exception that 2.0 mM BSO/24 hr reduced cell viability to approx.50%. However, alterations in either the cell doubling time(s) or the cell age density distribution(s) were not observed with the BSO exposures used to determine its radiosensitizing effect. BSO significantly radiosensitized hypoxic, but not aerobic, CHO cells when the GSH and NPSH concentrations were reduced to <10 and 20% of control, respectively, and maximum radiosensitivity was even achieved with ..mu..M concentrations of BSO. Furthermore, BSO exposure also enhanced the radiosensitizing effect of various concentrations of misonidazole on hypoxic CHO cells.

Research Organization:
Harvard Medical School, Boston MA
OSTI ID:
7054862
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 98:2; ISSN RAREA
Country of Publication:
United States
Language:
English

Similar Records

Effects of glutathione depletion by buthionine sulfoximine on radiosensitization by oxygen and misonidazole in vitro
Journal Article · Sat Jun 01 00:00:00 EDT 1985 · Radiat. Res.; (United States) · OSTI ID:5565481
Effects of D,L-buthionine-S,R-sulfoximine on cellular thiol levels and the oxygen effect in Chinese hamster V79 cells
Journal Article · Wed Aug 01 00:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States) · OSTI ID:6500430
Cellular glutathione depletion by diethyl maleate or buthionine sulfoximine: no effect of glutathione depletion on the oxygen enhancement ratio
Journal Article · Mon Oct 31 23:00:00 EST 1983 · Radiat. Res.; (United States) · OSTI ID:5011130

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANOXIA
ANTINEOPLASTIC DRUGS
AZOLES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMISTRY
CHO CELLS
DATA
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GLUTATHIONE
HAMSTERS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
INFORMATION
IONIZING RADIATIONS
MAMMALS
MISONIDAZOLE
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
RODENTS
SULFOXIDES
VERTEBRATES
X RADIATION