Effects of D,L-buthionine-S,R-sulfoximine on cellular thiol levels and the oxygen effect in Chinese hamster V79 cells

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(84)90325-0· OSTI ID:6500430

Astor, M B; Hall, E J; Biaglow, J E; Hartog, B

The role of glutathione (GSH) and total non-protein thiols (NPSH) in repairing radiation-induced free radical damage incurred under aerated and hypoxic conditions was investigated using Chinese hamster V79 cells cultured in vitro. GSH and NPSH levels were depleted in V79 cells of varying cell densities using the gamma-glutamyl-cysteine-synthetase inhibitor, D,L-Buthionine-S,R-sulfoximine (BSO). A small change in hypoxic cell radiosensitivity could be attributed to the loss of GSH while depletion of thiols to lower levels affected both aerated and hypoxic cell radiosensitivity, resulting in no change in the OER.

• Research Organization: Columbia Univ., New York, NY
• OSTI ID: 6500430
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 10:8; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
CELL KILLING
CHEMISTRY
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
FIBROBLASTS
GLUTATHIONE
HAMSTERS
IMINES
IONIZING RADIATIONS
MAMMALS
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN
OXYGEN ENHANCEMENT RATIO
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIATIONS
RADIOINDUCTION
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
THIOLS
VERTEBRATES
X RADIATION