Fixation of potentially lethal radiation damage in Chinese hamster cells by anisotonic solutions, polyamines, and DMSO
The effect of anisotonic solutions, dimethyl sulfoxide (DMSO), and polyamines on the fixation of potentially lethal damage (PLD) was examined. Exposure to anisotonic solutions after irradiation resulted in large decreases in cell survival in a radiation dose-dependent manner. Maximum increase in survival due to repair of PLD in V79 cells after a 1000-rad dose was about a factor of 4 while maximum decrease in survival due to fixation of damage was greater than 1000 times for some of the salt treatments tested. Irradiation at 0/sup 0/C resulted in more damage fixation by 0.05 or 1.5 M NaCl than irradiation at 37/sup 0/C. Fixation of PLD was also observed when cells were exposed to 1.0 or 1.0 M DMSO or 0.1 mM spermine or spermidine solutions. Fixation occurred in both exponentially growing cells and plateau-phase cells. The data demonstrate that a large amount of PLD which is normally not expressed can be converted to lethal damage by a variety of postirradiation treatments and that the PLD repair capacity of the cell may be very large.
- Research Organization:
- Atomic Energy of Canada Research Co., Pinawa, Manitoba
- OSTI ID:
- 6395302
- Journal Information:
- Radiat. Res.; (United States), Vol. 86:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DMSO
RADIOSENSITIVITY EFFECTS
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
HYPERTONIC SOLUTIONS
SPERMIDINE
SPERMINE
ANIMAL CELLS
AQUEOUS SOLUTIONS
BIOLOGICAL REPAIR
CELL CULTURES
COMPARATIVE EVALUATIONS
HAMSTERS
SURVIVAL CURVES
TEMPERATURE EFFECTS
X RADIATION
AMINES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
DISPERSIONS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MIXTURES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOLUTIONS
SOMATIC CELLS
SULFOXIDES
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)